Oligonucleotides of the general formula (I), in which: B is any nucleo base; A is O or CH2; X or Z are O, S, NH or CH2, where X and Z may be the same or different; V and W are an O, S, Se, NH2 or an alkyloxy radical, OH or SH, where V and W may be the same or different in a monomer unit; and L is an H atom or a partner of a bonding pair and C is -O-R and R is a possibly modified alkyl group with a...

The invention relates to novel antigens associated with Borrelia burgdorferi which are exported (or shed) in vivo and whose detection is a means of diagnosing Lyme disease. The antigens are extracellular membrane vesicles and other bioproducts including the major extracellular protein. The invention further provides antibodies, monoclonal and/or polyclonal, labeled and/or unlabeled, that react wit...

Compounds of formula (I), wherein A is a C1-C8 alkyl, perfluoroalkyl, cycloalkyl, aryl, substituted aryl, heterocyclic or substituted heterocyclic group; B is defined to include a variety of linking groups including straight and branched-chain alkylene and alkenylene groups as well as groups containing an ether, thio-ether, amine or amide group and various substituted and cyclic variations thereof...

Rare earth chelate-conjugated oligonucleotides useful in nucleic acid hybridization assays and for generating chelate-labelled probes of any desired sequence are disclosed. The particular class of chelates utilized exhibit an unusually high efficiency of rare earth element capture, correlated with emission of a high level of signal in time-resolved fluorescence spectroscopy compared to other struc...

A liquid pigmented (meth)acrylate-functional photocurable coating composition adapted for the secondary coating of optical glass fiber is disclosed. This photocurable composition has a pigment content which is sufficient to provide a coloration which can be distinguished from other colors and in the absence of a significant excess of said pigment, the particles of the pigment have a size of less t...

A method is provided for preparing a highly purified lysozyme dimer product which comprises dimerizing the lysozyme monomer with a suberimidate coupling reagent in a buffer solution adjusted to pH 10, stopping the dimerization at a given point by lowering the pH to 7 via addition of HCl or other suitable acid solution, and purifying the dimeric lysozyme by a series of elution steps carried out usi...

Process for generating and expanding cells having LAK cell activity comprises (a) treating human PBMC to deplete monocytes, (b) culturing the resulting peripheral blood lymphocytes in medium containing IL-2 and human plasma or serum to generate LAK activity and (c) expanding the cells by culturing at low cell density in medium containing IL-2 and human plasma or serum in a culture bag made from an...

A method for preparing purified ribonuclease dimers is provided which comprise the steps of dimerizing monomeric ribonuclease with a suberimidate coupling reagent, filtering out undimerized monomers and oligomers from the dimer solution using a series of ion exchange resin chromatography steps, monitoring the progress of the reaction through gel electrophoresis, and collecting the purified ribonuc...

Polypeptides are obtained on ribosomes in a cell-free translation system containing a matrix RNA and, as substrates, ATP, GTP and amino acids by means of a process during which translation products are generated in said system, which include the desired polypeptide, AMP, GDP, pyrophosphate and non-organic phosphate which are removed from the translation system as substrates are consumed, while ATP...

A transgenic animal model for viral infections, especially human immunodeficiency virus, is constructed by incorporating the genes ncoding essential components for viral infection of the animal into the chromosome of the embryo. Expression of the genes essential for infection and replication of the virus provides a means for assaying for compounds which inhibit infection and replication of the vir...

The gastrin releasing peptide receptor is solubilized and purified in an active form. The amino acid sequence and DNA encoding the receptor are provided.

A low calorie meat prod. comprises: (a) a mixt. comprising (1) comminuted lean meat substantially free from visible fat in an amt. of 20-95 wt.% of the mixt.; (2) a vegetable fat replacement ingredient comprising dietary fibre and starch in a wt. ratio of 1:32 to 1:1. The dietary fibre is at least 5 wt.% of the fat replacement ingredient dry matter when determined as non-starch polysaccharide (NSP...

Chromogenic derivatives of N-acetylneuraminic acid modified in the 4-position by eliminating the hydroxyl group, by replacing the hydroxyl group with fluorine, or by replacing the hydrogen of the hydroxyl group with methyl are used as substrates in colorimetric assays for human influenza neuraminidase activity in clinical specimens for the purpose of selectively diagnosing influenza infection. The...

PCT No. PCT/FR91/00028 Sec. 371 Date Jul. 8, 1992 Sec. 102(e) Date Jul. 8, 1992 PCT Filed Jan. 18, 1991 PCT Pub. No. WO91/10366 PCT Pub. Date Jul. 25, 1991.Skimmed milk is subjected to acidification by electrodialysis in one or two successive three-compartment electrodialyzers. The resulting milk is at a pH of 4.5 or 2.8. Milk at a pH of 4.5 may be used for obtaining refined casein as a substitute...

Oligonucleotides having tandem sequences of inverted polarity, i.e., oligonucleotides comprising regions of the formula: (1) or ng the nucleotide sequence of opposite polarity, are useful for forming an extended triple helix with a double-helical nucleotide duplex. The inverted polarity also stabilizes the single-strand oligonucleotides to exonuclease degradation.

A method of delivering a molecule of interest, which is a protein, a peptide, an oligonucleotide or a drug, into the cell, particularly into the cell nucleus and a molecule of interest-Tat protein conjugate which is useful in the method. The method and the conjugate are useful for diagnostic, prophylactic and therapeutic purposes.

A cellulase hydrolysate of tamarind polysaccharide is utilized as a substitute for a portion of metabolizable carbohydrates in processed foods to prepare reduced-calorie versions of said process foods having excellent organoleptic quality. The tamarind hydrolysate comprises DP 7 oligosaccharides, more typically, DP 7 and DP 9 oligosaccharides. The tamarind hydrolysate can be further processed util...

PCT No. PCT/AT91/00019 Sec. 371 Date Jul. 30, 1992 Sec. 102(e) Date Jul. 30, 1992 PCT Filed Feb. 5, 1991 PCT Pub. No. WO91/02186 PCT Pub. Date Aug. 22, 1991.To produce rottable thin-walled shaped bodies, such as cups, plates, trays, and the like, which have a high mechanical stability, in a baking process which is similar to that used to produce edible wafers, it is proposed in accordance with the...