Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. The reverse transcription reaction is performed in an appropriate buffer comprising a metal buffer which buffers the divalent cation concentration and which buffer preferably buffers both the pH and the divalent cation concentration. Said divalent cation is preferably Mn<2><+>. In a preferr...

The presence of amplified nucleic acids can be identified by detecting the presence of inorganic orthophosphate which is a stable by-product of the amplification process. There are a number of useful detection systems which can be used in either solution or dry formats. A preferred embodiment is to use a dry analytical element containing suitable reagents for generating a colorimetric signal in re...

The invention is to a polishing pad 14 that has a polishing surface 19 in which portions 17 and 18 of the polishing surface 19 have been removed. The removed areas 17 and 18 are annular rings adjacent an outer 15 and inner 16 edges of the polishing pad 14. The non-polishing surfaces 18 and 19 taper 17a and 18a downward from the polishing surface 19.

An apparatus for the cutting and mixing of fibrous products for the preparation of animal feed which includes a hopper where equipments for the cutting and mixing of fibrous product are installed. Based on this invention, such mixing and cutting equipment is constituted by one auger that is separated into two equal (welded) sections by a partition that is attached to the shaft of the auger itself....

The invention relates to a filter for separating solid particles from hot gaseous or liquid media which comprises a heatresistant carrier material of relatively large pore widths and a similarly heat-resistant material of smaller pore widths as a coating of the carrier material. Whereas the carrier material may for example be composed of ceramics, the coating layer is formed by a multiplicity of p...

A headbox tube bank apparatus and method of directing flow through same is disclosed for permitting the flow therethrough of stock in a papermaking machine. The apparatus includes a tube bank frame for rigidly supporting the tube bank, the frame defining a plurality of openings. A plurality of tubes cooperate with the frame such that each tube extends through and is supported by an opening. Each t...

PURPOSE:To easily obtain a connector having uniform, stable and high strength, to shorten a welding time, and to eliminate decomposition and deterioration of a resin by fusion-bonding a welding rod in which two opposed contact surfaces are press-bonded and which has a triangular shape or similar sectional shape in a wedge state between the connecting members. CONSTITUTION:In the case of welding wi...

PURPOSE:To obtain a joint surface having uniform, stable and high strength and to shorten a heating time by providing a contact surface of a platelike heating block with a melted contact position, a welding rod guiding groove, welding rod feed guide means, a pressing means for press-bonding a welding rod to the melted contact position, and an electrically heating means. CONSTITUTION:A heating bloc...

PCT No. PCT/US92/05225 Sec. 371 Date Dec. 16, 1993 Sec. 102(e) Date Dec. 16, 1993 PCT Filed Jun. 25, 1992.A powder dispenser 10 includes a powder housing 28 for holding a supply of powdered material to be dispensed, the powder housing including a first conduit 32 extending there through in displaced relation to the powdered material; a metering plate 16 having only a single receptacle area 24 for ...

A process is described for the production of a hydroxylic compound selected from alcohols and diols by hydrogenation of a hydrogenatable material selected from monoesters of carboxylic acids, monoesters of dicarboxylic acids, diesters of dicarboxylic acids, aldehydes, olefinically unsaturated aldehydes, and mixtures of two or more thereof, which process comprises the steps of: (a) providing a hydr...

A transmitting power control unit which generates and transmits a wideband transmission signal with a power level which does not differ by frequency. This transmitting power control unit includes means for generating voltage corresponding to a frequency of a transmission signal. The power of the transmission signal is controlled by added the voltage which is obtained by adding the generated voltag...

PCT No. PCT/FI94/00393 Sec. 371 Date May 2, 1995 Sec. 102(e) Date May 2, 1995 PCT Filed Sep. 5, 1994 PCT Pub. No. WO95/07573 PCT Pub. Date Mar. 16, 1995An arrangement for controlling the operation of a high-frequency power amplifier includes a high-frequency power amplifier connected to a forward line and a control, and an attenuator connected to the input side thereof. A sampler and a detector ar...

A method of baling straw, hay and like materials by feeding wrapping material around a cylindrical bale to hold the bale together is provided, in which method the wrapping material comprises a plurality of ends of twine which are fed simultaneously around the bale at positions spaced apart axially along the bale. The wrapping material comprises a baler twine package comprising a plurality of spool...

A ski boot having a shell surmounted by an upper having a rear opening for passage of the foot, and at least one tightening system of the upper on the lower part of the leg, a flexion control device both of amplitude and force of the upper in the frontward direction being located at the rear of the boot. The flexion control device includes an energy flap affixed to the shell base on which it is jo...

A gas sensor having a large operation current and being operated at a relatively low temperature includes a substrate having a first surface and a second surface. A sensing area is provided on the first surface for increasing the conductivity across it when the sensing area detects a certain gas. A controlling area is provided on the first surface and beside the sensing area for permitting a tunne...